Interleukin-11 Drives Early Lung Inflammation during Mycobacterium tuberculosis Infection in Genetically Susceptible Mice
Figure 4
Protein levels of IL-11 affect IL-11 mRNA expression.
(A) In vivo administration of anti-IL-11 antibodies leads to a selective down-regulation of IL-11 mRNA. The level of expression was quantified in 5 individual mice per group, using qrt-PCR and normalization against the level of GAPDH expression. Results obtained in 1 of 2 similar experiments are expressed as mean ± SEM (for IL-11 expression P = 0.021, for other cytokines P>0.05). (B) Introduction of 100 ng/ml rIL-11 in cultures of lung cells up-regulates the expression of IL-11 mRNA. Results of two similar experiments are expressed as mean of 3 wells ± SEM (P<0.01, ANOVA, compared to negative controls and cultures stimulated with 10 ng/ml IL-11).