Formin1 Mediates the Induction of Dendritogenesis and Synaptogenesis by Neurogenin3 in Mouse Hippocampal Neurons
Figure 3
Effects of Fmn1-Ib overexpression on the morphology and synaptology of cultured hippocampal neurons.
Cells were tranfected at 3 DIV with an EGFP (A and C) or EGFP-Fmn1-Ib (B and D) expressing C2 vector and immunostained with antibodies against GFP and VGlut1 or VGAT. (A–D) Representative immunofluorescence images of neurons marked in green for GFP and with its synaptic contacts marked in red. Scale bar, 25 µm. Lower panels show the boxed regions at higher magnification. (E) Number of primary dendrites. (F) Counts of VGlut1 immunoreactive terminals in contact with a neuron within a circular region of interest (ROI) with a diameter of 50 µm and centered in the neuronal soma. (G) Counts of VGAT immunoreactive terminals in contact with a neuron per ROI. Typically 60–75 neurons were evaluated in each condition (n = 3). (H) Ratio of excitatory/inhibitory (E/I) synaptic terminal number. Data are mean ± SEM and significance levels were determined using a Student t-test; ** p<0.01, *** p<0.001 versus EGFP expressing neurons values.