Identification of Novel Linear Megaplasmids Carrying a ß-Lactamase Gene in Neurotoxigenic Clostridium butyricum Type E Strains
Figure 5
PFGE of undigested genomic DNA of strains ISS-145/1 (lane 1); ISS-20 (lane 2); ISS-21 (lane 3); ISS-109 (lane 4); ISS-86 (lane 5); ISS-190 (lane 6); KZ-1886 (lane 7); KZ-1890 (lane 8); LCL-063 (lane 9); LCL-155 (lane 10).
S.e., XbaI-digested genomic DNA fragments of Salmonella enterica serotype Braenderup strain H9812 (ref. 34). PFGE conditions: 5–60 s pulse at 6 V/cm for 18 h (5a). Southern hybridization with a bont/E gene probe showing that the gene probe hybridized to the chromosome bands of strains (5b). Southern hybridization with a β-lactamase gene probe showing that the gene probe hybridized to the megaplasmids of strains ISS-145/1 (lane 1); ISS-20 (lane 2); ISS-21 (lane 3); ISS-109 (lane 4); ISS-86 (lane 5); ISS-190 (lane 6); LCL-063 (lane 9); LCL-155 (lane 10) (5c).