A High Resolution Genome-Wide Scan of HNF4α Recognition Sites Infers a Regulatory Gene Network in Colon Cancer
Figure 11
HNF4α protein expression and DNA binding activity with nuclear extracts isolated from Aroclor 1254 treated Caco-2 cells.
a) HNF4α Western blotting of 20 µg Caco-2 cell extract. A clear induction of HNF4α protein expression was seen after 48 h and 72 h of Aroclor1254 treatment. b) Electrophoretic mobility shift assays with 2.5 µg Caco-2 cell nuclear extract and oligonucleotides corresponding to the A-site of the HNF1α promoter (HNF1pro) as 32P labeled probe. In supershift assays an antibody directed against HNF4α (+) was added. Binding of HNF4α was significantly increased after 72 h of Aroclor1254 induction.