A High Resolution Genome-Wide Scan of HNF4α Recognition Sites Infers a Regulatory Gene Network in Colon Cancer
Figure 5
Promoter-proximal ES of HNF4α.
a) Bootstrapping analysis of HNF4α binding motif (matrix M01031) in promoter-proximal and promoter distal regions. 100 promoter-proximal ES (−138 to −2 relative to the TSS) were compared to 100 promoter-distal ES (−24972 to −23489 relative to the TSS) by the bootstrapping analysis tool POBO [48]. Promoter-proximal ES show a significantly lower number of HNF4α motifs. b) ES (300 bp surround the peak position) were sorted by their P-value (as calculated by the MAT algorithm) and divided into bins of 1,000 ES. For each bin the number of HNF4α motif occurrences and the percentage of promoter-proximal ES were calculated. As can be seen, ES with a high P-value (weak ES) are more likely to be located promoter-proximal but to contain no HNF4α binding motif.