Regulation of Gene Expression in Plants through miRNA Inactivation
Figure 3
Effect of stoichiometry on activity of two miRMON1 decoys (bulged, miRMON1_3 B decoy, and mismatched, miRMON1_2 M decoy).
Diagram of miRMON1_3B and 2M decoys embedded in the 3′ UTR of a GUS coding transcript and expression of miRMON1-targeted GFP reporter co-expressed with various concentrations of miRNA decoys in N. benthamiana leaves. Leaves were co-transformed with one part GFP reporter, 5 parts miRMON1, and variable parts miRNA decoy. Agrobacterium transformed with an empty vector was added to achieve a total OD600 = 1 of Agrobacterium for each transformation. GFP expression was measured by fluorescence microscopy and protein blot. The Rubisco band visualized by Ponceau staining shows the loading control. Relative GFP expression normalized to Rubisco and relative GUS expression as measured by qRT-PCR are indicated.