Cutaneous Squamous Cell Carcinoma (SCC) and the DNA Damage Response: pATM Expression Patterns in Pre-Malignant and Malignant Keratinocyte Skin Lesions
Figure 2
Fluorescent pATM staining of NHPK +/− UVB.
Same cells as in Fig 1 fluorescently labelled using DAPI (blue) to stain the nucleus. pATM was fluorescently labelled with Alexa Fluor 568 (red) and cells visualised using confocal microscopy. Findings mirror those observed in Fig 1 - a very specific pattern of cytoplasmic staining is seen; at 60 minutes following UVB there is more nuclear expression of pATM which is short-lived, returning back to the cytoplasm after 2 hours.