Regulation of rDNA Transcription by Proto-Oncogene PELP1
Figure 5
CDKs regulate PELP1 nucleolar localization.
(A) ZR-75 cells at sub confluence were fixed and the localization of phos991PELP1was analyzed by using confocal microscopy. (B) Localization of GST-tagged PELP1 or PELP1 S991AMT or PELP1 S991EMT were visualized by using confocal microscopy with the antibody against the GST epitope in ZR-75 cells. (C) PELP1 was visualized in ZR-75 cells after serum starved for two days and released with 10% serum in absence or presence of roscovitine (10 µM) by using confocal microscopy. (D) 293T cells were transfected with pHrD luciferase reporter vector along with PELP1 WT or PELP1 S991AMT or PELP1 S991EMT expressing vectors. Cells were serum starved for 24 h and then stimulated with 10% serum for 24 h and the reporter gene activity was measured. Results are the average of 3 independent experiments. *p-value <0.05.