Impaired Sprouting and Axonal Atrophy in Cerebellar Climbing Fibres following In Vivo Silencing of the Growth-Associated Protein GAP-43
Figure 6
Morphometric analysis in adult mice confirms CF atrophy after GAP-43 silencing.
Representative confocal images of mouse parasagittal cerebellar sections, immunolabelled for VGLUT2 (in red; A–B). CFs were transduced with control (A) and siGAP sequences (B). (C) Total length and (D) density of varicosities per length unit are reduced in siGAP CFs compared to control (N = 19 and 15, from 3 animals per group, mean ± SEM).