HuB (elavl2) mRNA Is Restricted to the Germ Cells by Post-Transcriptional Mechanisms including Stabilisation of the Message by DAZL
Figure 4
Cis-regulatory elements control differential HuB mRNA stability in the soma and germ cells.
(A) 3′UTR sequence of the B deletion reporter. Base substitutions were made in 10 nucleotide blocks to create the B substitution mutants Bs1–Bs14. Each 10 nucleotide block was substituted with the sequence GAGGATCCGA. (B) Expression of EGFP and mCherry protein in embryos injected with the indicated Bs substitution mutant reporters. 200 pg of each reporter was injected. All EGFP images were taken at 2000 ms, and all mCherry images were taken at 150 ms. Higher magnification images of mCherry expression in the germ cells are shown as insets. (C) Whole-mount in situ hybridisation using an antisense probe directed against the mCherry coding sequence. Higher magnification images of germ cells are shown as insets.