Communication Impairments in Mice Lacking Shank1: Reduced Levels of Ultrasonic Vocalizations and Scent Marking Behavior
Figure 1
Analysis of ultrasonic vocalizations.
Energy within the spectrum is shown by time (A) and frequency (B) and time x frequency (C, reflected as “darkness”). Peak amplitude, i.e. loudness, was defined as the point with the highest energy within the spectrum (“darkest” points over time in C). Peak frequency was defined as the frequency at the location of the peak amplitude within the spectrum (“darkest” points over time in C). Peak frequency and peak amplitude were derived from the average spectrum of the entire call, meaning that values obtained per time point were averaged over time. The extent of frequency modulation was defined as the difference between the lowest and the highest peak frequency within each call, i.e. derived from the non-averaged call (C). Temporal parameters were latency to start calling, total calling time, call duration (C).