MMP-2 siRNA Inhibits Radiation-Enhanced Invasiveness in Glioma Cells
Figure 6
p-MMP-2 inhibits colony formation and induces apoptosis and activation of pro-apoptotic molecules in glioma cells.
A, Clonogenic assay was performed as described in Materials and Methods. The cells were cultured, and colonies larger than 50 cells were counted. Columns: mean of triplicate experiments; bars: SD; *p<0.01, significant difference from radiation. B, U-251 and U-87 cells were transfected with mock, p-SV or p-MMP-2, and irradiated as described earlier. After 24 h of radiation, cells were evaluated with the TUNEL assay according to manufacturer's instructions and photographed under fluorescent microscopy. Apoptosis was measured by counting the percentage of cells that showed DNA staining in five different fields in each group. Columns: mean of triplicate experiments; bars: SD; *p<0.01, **p<0.001, significant difference from mock, p-SV or irradiated controls. C, Equal amounts of protein from whole cell lysates of control and treated cells were analyzed by Western blotting using caspase-specific primary antibodies. GAPDH served as a loading control.