Quantitative Whole Body Biodistribution of Fluorescent-Labeled Agents by Non-Invasive Tomographic Imaging
Figure 6
Comparison of the kinetics of whole body fluorescence changes for four different NIR-labeled agents.
Four different agents were injected IV into nu/nu mice (n = 3 mice/group), and animals were imaged immediately and at 1, 3, 6, 12, 24, and 100h post injection by FMT2500. A, Whole body fluorescence was quantified at each time point using ROIs to capture the torso and head. Data was normalized as the percentage of the injected dose. B, Plasma pharmacokinetics data was collected in separate sets of animals (as described in the Materials and Methods) to provide data regarding circulating agent kinetics. Data was normalized as the percentage of the injected dose. C, An approximation of the kinetics of agent accumulation in tissue was established by subtracting the normalized plasma pharmacokinetic profiles from the whole body fluorescence profiles, making the assumption for each agent that the earliest imaging time point represented 100% of the agent in circulation. Corrected results reveal differences in extravasation and whole-body tissue clearance rates for all four agents.