Breakdown of the Blood-Brain Barrier during Tick-Borne Encephalitis in Mice Is Not Dependent on CD8+ T-Cells
Figure 6
Measurement of BBB permeability using sodium fluorescein in CD8α−/− mice.
Mice were inoculated subcutaneously with TBEV (100 pfu). BBB permeability is expressed as brain:serum ratio (%). (A) Thirty minutes after intraperitoneal injection with sodium fluorescein, the sera and brains were processed as described in the Material and Methods section and the fluorescence was measured. Each line segment represents the mean of four samples (points). The increase in brain:serum fluorescence in infected versus uninfected mice was statistically significant (p<0.05). (B) Sodium fluorescein accumulation in the brains of CD8α−/− before infection and at the terminal phase (i.e. phase with neurological signs) of TBE. The brains were photographed under UV illumination.