Cancer Induces Cardiomyocyte Remodeling and Hypoinnervation in the Left Ventricle of the Mouse Heart
Figure 5
Immunohistochemistry of nerve fibers.
Nerve fibers were detected by immunostaining for protein gene product 9.5 (PGP9.5). Scale bar = 50 µm. Nerve fibers (arrows) were counted in an unbiased counting frame. The dashed line is the inclusion line, the closed line and its extensions represents the exclusion line (see Methods section).