Live Tissue Imaging Shows Reef Corals Elevate pH under Their Calcifying Tissue Relative to Seawater
Figure 2
In vivo images of the calicoblastic epithelium of S. pistillata.
(A) confocal optical section at 1 µm above the coverslip. Yellow = intracellular fluorescein diacetate (FDA) staining the calicoblastic epithelium. (B) Transmitted light image merged with (A) to show crystal detail. (C) Profile view showing vertical relief of (A) acquired by Z-stack reconstruction. (D) confocal optical section at 1 µm above the coverslip. Green = calcein in the extracellular subcalicoblastic medium and staining crystals. Crystals are stained more brightly than the subcalicoblastic medium. (E) Transmitted light image merged with (D) to show position of tissue. C = crystals. S = fluid-filled space under the calicoblastic epithelium. M = sample margin. CE = calicoblastic epithelium. X = position of Z-stack vertical reconstruction shown in (C).