Antisense-Mediated Knockdown of NaV1.8, but Not NaV1.9, Generates Inhibitory Effects on Complete Freund's Adjuvant-Induced Inflammatory Pain in Rat
Figure 1
Expression profile of NaV1.8 and NaV1.9 at mRNA and protein levels following CFA treatment.
(A) Representative RT-PCR results of five isoforms of voltage-gated sodium channels. Amplicons of NaV1.1, NaV1.6, NaV1.7, NaV1.8, NaV1.9 and ß-actin were 540 bp, 509 bp, 441 bp, 515 bp, 572 bp and 229 bp, respectively. (B) Averaged fold changes of mRNA expression as normalized with naive control (n = 3). (C) Double immunofluorescent labeling of DRG neurons by anti-NaV1.8 (red) and anti-NF-200 (green) or anti-NaV1.9 (red) and anti-NF-200 (green) antibodies. (D) The percentage of Nav1.8- and Nav1.9-positive profiles as a proportion of the total number of DRG neurons before and after CFA treatment (n = 8). (E) Western blotting examples of NaV1.8 and NaV1.9 in naive and CFA-treated DRG neurons. (F) Averaged protein expression of NaV1.8 and NaV1.9 between naive and CFA-treated DRGs (normalized with the internal control tubulin) (n = 3 for each group). CFA, complete Freund's adjuvant. * p<0.05, **p<0.01, ***p<0.001.