IFI16 Induction by Glucose Restriction in Human Fibroblasts Contributes to Autophagy through Activation of the ATM/AMPK/p53 Pathway
Figure 3
Glucose restriction decrease cell survival.
(A) Sub-confluent cultures of young WI-38 HDFs were incubated with the indicated concentrations (mM) of glucose in the medium supplemented with 10% fetal bovine serum for 24 h. At the end of the incubations, cells were photographed using a phase contrast microscope. (B) At the end of the incubation of HDFs shown in the panel (A), the number of viable cells were counted (in triplicates) by Trypan Blue exclusion method using the Countess Automated Cell Counter (Invitrogen). (C and D) HDFs (in triplicates) were grown in 96-well plates and cells were incubated at the indicated concentrations of glucose for either 24 h (white bars) or 48 h (black bars). At the end of the incubations, cell viability and the extent of apoptosis were determined using ApoTox-Glo Triplex Assay (Promega) as described in the method. Experiments were repeated three times, which gave similar results.