Dose-Dependent Onset of Regenerative Program in Neutron Irradiated Mouse Skin
Figure 3
Immunoreactivity for KRT6 and KRT10.
Representative images are reported in Panel A for Krt6, and Panel C for Krt10. Scale black bar represents 20 µm. Abbreviations: N = negative controls obtained by omission of the primary antibodies; U = untreated sham irradiated samples; 6, 24 = hours of sampling after irradiation, respectively with 0.2 and 1 Gy of 14 MeV neutron irradiation. Krt6 was cytoplasmic accumulated at the lower dose of 0.2 Gy group (panel A), with a thickening of epidermis as compared with sham and 1 Gy irradiated mice. Arbitrary densitometric units of protein expression are plotted as whisker box in panel B, and showed limited individual variation within the groups. Statistical analysis showed a significantly (p<0,05) higher level for the group of mice treated with 0.2 Gy irradiation (*). KRT10 did not show a statistically significant variation among the experimental mice (Panel C). Arbitrary densitometric units of protein expression are plotted as whisker box in panel D.