Staphylococcus aureus Protein A Binds to Osteoblasts and Triggers Signals That Weaken Bone in Osteomyelitis
Figure 2
Staphylococcus aureus SpA binds to osteoblast tumour necrosis factor receptor 1.
(A) S. aureus Newman, Newman pCU1spa and Newman pCU1spa L17A were lysed, separated on 7.5% SDS-PAGE gels, electroblotted onto PDVF membranes. Membranes were probed with either a nonspecific IgG or anti-SpA IgY antibody. Protein bands were detected using species specific horseradish peroxidase-conjugated secondary antibody and chemiluminescence. (B) Osteoblasts (5×105 cells/ml) were preincubated with either isotype control antibody or inhibitory monoclonal antibody against TNFR-1 for 15 mins prior to addition to S. aureus Newman pCU1spa L17A. Adhesion was determined by measuring the intracellular enzyme alkaline phosphatase content at 405 nm in a microplate reader. *P<0.05, n = 7.