A Sodium Leak Current Regulates Pacemaker Activity of Adult Central Pattern Generator Neurons in Lymnaea Stagnalis
Figure 6
Gd3+ partially blocked ILeak via the U-type channels in RPeD1 neurons.
(A) ILeak conducted by the U-type channel is partially blocked by Gd3+. Representative ILeak (A1) and ILeak - voltage (I–V) relations (A2) of naïve control, control dsRNA/siRNA, and U-type dsRNA/siRNA 1&2 in saline with or without 10 µM Gd3+. (B3) Average normalized ILeak in 10 µM Gd3+ over saline at -160 mV, −110 mV, and −60 mV for naïve control (n = 4), control dsRNA (n = 4), U-type dsRNA (n = 3), control siRNA (n = 4), U-type siRNA 1 (n = 4), and U-type siRNA 2 (n = 4). The dashed line represents the current activity in saline. 10 µM Gd3+ reduced significantly the ILeak in the naïve control and control dsRNA/siRNA groups, but not in U-type dsRNA/siRNA groups. Data are presented as mean ± SEM. Significant difference (P<0.05) between Na+ free saline current normalized to saline is denoted by *. (B) 10 µM Gd3+ hyperpolarized the membrane potential. (B1) Representative records of the membrane potentials of RPeD1 from naïve control, control dsRNA/siRNA, and U-type dsRNA/siRNA group. 1 & 2 indicate perfusion with and without 10 µM Gd3+, respectively. (B2) Average difference of the membrane potential by 10 µM Gd3+ in naïve control (n = 4), control dsRNA (n = 3), U-type dsRNA (n = 4), control siRNA (n = 3), U-type siRNA 1 (n = 4), U-type siRNA 2 (n = 3) groups. * and † indicate the significant difference (P<0.05) between naïve control and U-type RNAi treatment and between control RNAi and U-type RNAi treatment, respectively.