Temperature-Induced Protein Secretion by Leishmania mexicana Modulates Macrophage Signalling and Function
Figure 6
Modulation of translocation of transcription factors AP-1 and NF-κB following exoproteome incubation.
B10R macrophages were untreated (Nil), infected with L. mexicana or incubated with exoproteome for 16 h and stimulated with 100 ng/ml of LPS for 1 hour the following day. (A) AP-1 is degraded after infection and exoproteome incubation. No increase in translocation in response to LPS can be seen. (B). A noncanonical form of NF-κB translocates to the nucleus following LPS-stimulation of infected macrophages (p35/p50). Induction of this form of NF-κB can also be seen after stimulation of the exoproteome-treated macrophages. Furthermore, induction of the normal p65/p50 in response to LPS is greatly reduced compared to Nil. S, specific competition (100-fold excess of specific nonradioactive oligonucleotide incubated with nuclear proteins of Nil+LPS); NS, nonspecific competition (100-fold excess of nonspecific, nonradioactive SP-1 oligonucleotide incubated with nuclear proteins of Nil+LPS).