The Rate of NF-κB Nuclear Translocation Is Regulated by PKA and A Kinase Interacting Protein 1
Figure 9
Disruption of the interaction between AKIP 1A and PKAc decreases phosphorylation of p65 at serine 276 in the cytosol.
HeLa cells were transfected with CAT 1-29 (lanes 1 and 2), AKIP 1A (lanes 7 and 8), or CAT 1-29 and AKIP 1A (lanes 5 and 6) in the absence (lanes 1, 3, 5, 7) or presence (lanes 2, 4, 6, 8) of TNFα/8-CPT-cAMP. Samples were fractionated and the cytosolic fraction analyzed on 10% SDS-polyacrylamide gels and Western blotted with antibodies to phospho-Ser 276 p65 and p65. Lines indicate duplicate samples.