Cholinergic Modulation of Narcoleptic Attacks in Double Orexin Receptor Knockout Mice
Figure 7
Neostigmine microinjection sites into the PnO/PnC increased behavioral arrests in DKO but not WT mice.
(A). Mean (± SEM) time per mouse spent in full behavioral arrests are plotted in 30 min bins across the entire recording period following PnO/PnC microinjections of ACSF (open squares), neostigmine (closed triangles) and neostigmine+atropine (open triangles). Grey area indicates dark phase. (B) Mean (± SEM) time per mouse spent in arrest over the entire recording following ACSF, neostigmine and neostigmine+atropine. Neostigmine increased the time spend in arrest compared to ACSF and neostigmine+atropine, * denotes p<0.05 based on post-hoc testing following a significant two-way ANOVA (p<0.0002). The difference between ACSF and neostigmine+atropine was not significant (p>0.05). (C) Cumulative distributions of arrest bout duration over the entire of recording. Distributions following ACSF, neostigmine or neostigmine+atropine were not different as compared using the Kolmogorov-Smirnoff statistics. (D) Mean (± SEM) time spent in the inactive state. (E) Mean (± SEM) time spent wheel running. Values in D and E were not statistically different across microinjection conditions (p>0.05, 2-way ANOVAs).