Small Molecule Amiloride Modulates Oncogenic RNA Alternative Splicing to Devitalize Human Cancer Cells
Figure 4
Phosphorylation of Akt kinase (activation) and PP1 phosphatase (inactivation) in relation to amiloride-altered AS mechanisms.
(Panel A). Western blots show significant decreases of cytoplasmic p-Akt, nuclear p-Akt and nuclear p-PP1 in Huh-7 cells treated with amiloride at 0.3 mM and above. (Panel B) RT-PCR and western blot analyses show that pre-treatment with a PP1 inhibitor, okadaic acid, abrogated the amiloride effects on the BCL-X and HIPK3 oncogenic RNA splicing, the phosphorylation of ASF2/ASF splicing factor and the level of SRp20 in Huh-7 cells.