Rgs2 Mediates Pro-Angiogenic Function of Myeloid Derived Suppressor Cells in the Tumor Microenvironment via Upregulation of MCP-1
Figure 3
Lack of Rgs2 in endothelial cells does not affect migration, BrdU incorporation, or tube fomation.
Lung microvascular endothelial cells were isolated from lungs of wild type and Rgs2−/− mice and cultured. (A) 1×105 cells were placed in the top chamber of a Transwell, and migrated to growth medium for 4 hours. Cells on the bottom of the Transwell (migrated cells) were fixed, stained with crystal violet, and counted. Experiment was performed 3 times in duplicate. (B) Cells were pulsed with 10 uM BrdU for 2 hours, then collected, processed, and analyzed by flow cytometry. The average percentages of BrdU positive cells was plotted. Experiment was performed 2 times in duplicate. (C) Cells were seeded on top of Matrigel in growth medium and allowed to form tube structures for 48 hours. Branch points were counted, and the average number per field was graphed.