Early Growth Response Gene-2 (Egr-2) Regulates the Development of B and T Cells
Figure 1
Defective thymus development in Egr-2 transgenic mice.
A). confirmation of presence of transgene by PCR. B). expression of Egr-2 in thymocytes from wild type, Egr-2 cKO and two Egr-2 cTg mouse lines (14 and 18) determined by quantitative RT-PCR. The relative expression was measured after normalization against b-actin. * Egr-2 was not detected in Egr-2 cKO T cells. C). Thymus size in wild type, Egr-2 cTg and Egr-2 cKO mice. D). Thymocytes from wild type and Egr-2 transgenic mice were stained with anti-CD4 and anti-CD8. E). Expression of CD3, TCRβ and CD5 by gated CD4+ or CD8+SP T cells in thymus. The data are representative of three experiments in which 3 to 5 mice from each group were used.