Saccharomyces boulardii Improves Intestinal Cell Restitution through Activation of the α2β1 Integrin Collagen Receptor
Figure 4
Sb supernatant but not Sc supernatant improves mouse enterocyte migration along the crypt-villus axis.
Mice were daily force-fed for one week with unused culture medium for Sb (control), Sb supernatant (Sb sn) or Sc supernatant (Sc sn) then injected with BrdU 24 hours before sacrifice. Small intestinal tissues were processed for immunostaining. The BrdU-labeled enterocyte migration along the crypt-villus axis was traced on tissue sections by immunostaining with an anti-BrdU Ab (A, B and C) and quantitatively assessed (D) as described in Methods. (n = 5). ** P<.01. Arrow: basal site of the enterocytes. Scale bar: 150 µm.