Single Gene Deletions of Orexin, Leptin, Neuropeptide Y, and Ghrelin Do Not Appreciably Alter Food Anticipatory Activity in Mice
Figure 4
Calorie restriction of leptin knockout mice.
Measure of fraction of time engaged in activity during the anticipatory period prior to feeding (ZT 7–9). Beginning from day 7, WT AL and WT CR mice have statistically significant differences in fraction of time spent engaging in (A) total high activity behaviors (sum of hang, jump rear, and walk) as well as individual behaviors such as (C) hang, (E), jump, (G) rear, and (I) walk. In contrast, Leptin KO mice on AL and CR feeding have no statistical difference in (B) total high activity behaviors. (D) Hang reaches statistical significance at day 14 that is sustained through day 28 and (F) jump reaches transient statistical difference at day 14 and 28. (H) Rear and (J) walk is not statistically different between AL and CR. Statistics were performed using the Mann-Whitney Test with post-test, * = p<0.05; ** = p<0.01; and *** = p<0.001. Error bars represent IQR. n = 8 WT AL; n = 8 WT CR; n = 7 KO AL and n = 8 KO CR.