Single Gene Deletions of Orexin, Leptin, Neuropeptide Y, and Ghrelin Do Not Appreciably Alter Food Anticipatory Activity in Mice
Figure 2
Calorie restriction of orexin knockout mice.
(A) Body weights of orexin KO and WT mice on AL and CR feeding conditions. (B) Percent gain and loss of weight relative day 0 of the experiment. Orexin KO mice are resistant to weight loss when compared with WT CR mice (C) Fraction of time engaged in high activity behaviors (sum of hanging, jumping, rearing and walking) observed on day -7. (D) Fraction of time engaged in food bin entry, (E) fraction of time engaged in hanging, (F) fraction of time engaged in jumping, (G) fraction of time spent rearing, (H) fraction of time spent walking, and (I) fraction of behavior of spent in a resting state on day -7 of the experiment. n = 12 orexin KO and n = 23 orexin WT. (J) High activity behaviors during the 3 hrs prior to scheduled meal delivery (ZT 7–9) for WT mice on AL (n = 18) and CR (n = 9) and (K) Orexin KO mice on AL and CR (n = 6 for both groups). Statistics were performed using Mann-Whitney Test * = p<0.05; ** = p<0.01; *** = p<0.001. Error bars represent the IQR for behavioral data and SEM for body weight data.