Single Gene Deletions of Orexin, Leptin, Neuropeptide Y, and Ghrelin Do Not Appreciably Alter Food Anticipatory Activity in Mice
Figure 1
Comparison of behavior and body temperature under temporally and calorically restricted feeding conditions.
(A) Mean daily temperature for each group of mice during the experiment. The bold line represents the median of the mean daily temperatures for individual mice, and the shaded regions represent the IQR. (B) Food intake for each group of mice. The line represents the mean and the bars represent the SEM. (C) Temperature and high activity seconds per hr seven days prior to beginning the special feeding regimen. Median temperature is represented by the filled circles in the top half of the panel (corresponding to the right y-axis) and median high activity is represented by the solid line in the bottom half of the panel (corresponding to the left y-axis). The bars represent IQR. (D) Temperature and high activity seconds per hourly bin seven days after the start of the special feeding regimen. (E) Temperature and high activity seconds per hourly bin 14 days after the start of the special feeding regimen. (F) Temperature and high activity seconds per hourly bin 21 days after the start of the special feeding regimen. (G) Seconds of high activity observed during the three hours prior to feeding (ZT 4–6) for the duration of the experiment. Medians and IQR are shown. (H) Seconds of high activity observed during the 3 hrs prior to feeding (ZT 4–6) divided by total seconds of high activity during the entire recording period. * = p<0.05, ** = p<0.05, *** = p<0.001 (Kruskal-Wallis Test with Dunn's Post Test). n = 9 for AL, n = 8 for CR, and n = 6 for RF.