Transglutaminases (TGs) in Ocular and Periocular Tissues: Effect of Muscarinic Agents on TGs in Scleral Fibroblasts
Figure 7
Transamidase activity of TG-2 with stimulation of atropine and carbachol in scleral fibroblasts.
Line graph illustrating the results of the transamidase activity of TG-2 in 100 µg of protein lysate from mouse and human cultured scleral fibroblasts. Values were normalized against control values. The mouse and human scleral fibroblasts treated with carbachol and atropine at 0.01, 0.1, 1, 5 and 10 µM for 5 days. The SF treated with carbachol increased the transamidase activity of endogenous cellular TG-2 but this activity was reduced by atropine treatment in a concentration-dependent manner. The values represented the means of three independent samples and error bars represent standard deviation, n = 3, *p<0.05, **p<0.01. Straight lines represent the mouse SF and dashed lines represent the human SF. Open markers are atropine treated and closed markers are carbachol treated SF cells.