Further Improvements of the P. falciparum Humanized Mouse Model
Figure 2
Effect of the aging process on the production of cytokines in NOD/SCID mice.
Panel (A): levels of various inflammatory mediators (IL-6, MCP-1, TNFα, IFNγ) in supernatants of peritoneal cells (n = 10) and splenocytes (n = 16) from young vs aged mice after stimulation with LPS (1 µg/ml) ; Panel (B) : levels of reactive oxygen intermediates, measured by chemiluminescence, in the presence of PMA (10 µg/ml) produced by peritoneal cells (n = 4) and splenocytes (n = 5) taken from young vs aged mice; Panel (C): in vivo levels of IFNγ in aged mice (with long-lasting parasitemia) (n = 4) compared to young mice (with short-lived parasitemia) (n = 5) as measured in serum samples collected 1, 3, and 6 days post infection with P. falciparum. Cytokine levels were measured by using the CBA mouse inflammatory kit by FACS. Results represent the mean ± SEM from two different experiments.