ETS1 Mediates MEK1/2-Dependent Overexpression of Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) in Human Cancer Cells
Figure 5
Characterization of MEK kinase responsive region on the CIP2A promoter.
A. Luciferase assay showing the activity of different length CIP2A promoters when treated with DMSO and U0126 (10 uM) for 24 h, identifying the MEK responsive region to lie between −672 bp to −27 bp of the CIP2A promoter. B. Luciferase assay showing the basal activity indicated CIP2A promoters. C. Luciferase assays showing the activity of different length CIP2A promoters when treated with DMSO and U0126 (10 uM) for 24 h, further narrowing down the MEK responsive region to be between −108 bp and −27 bp of the CIP2A promoter (*, P<0.05; **, P<0.00; n.s. = non-significant). Shown is the Mean+SD from three independent experiments.