Differential Expression of Type III Effector BteA Protein Due to IS481 Insertion in Bordetella pertussis
Figure 6
Construction and characterization of B. pertussis BteA mutants.
(A) Physical map of the btcA−bteA region of BteA mutants derived from B. pertussis BP155 (vaccine-type) and BP157 (nonvaccine-type). WT, wild-type; ΔbteA, a 178-bp deletion around the 5′ region of bteA; ΔIS481, IS481a deletion from the bteA 5′-UTR; +IS481, IS481a insertion in the bteA 5′-UTR. (B) Expression of BteA protein in the BteA mutants. The mutants were cultured in modified SS medium for 24 h. Total protein from the bacterial cells (Cell) and culture supernatants (CS) was analyzed with immunoblot using anti-BteA antiserum.