Prostaglandin E2 Signals Through PTGER2 to Regulate Sclerostin Expression
Figure 6
Reductions in MEF2 expression do not impair PGE2 decrease of Sost.
(A) UMR106.01 cells were cultured with 50 nM of scrambled or Ptger4 siRNA for 48 hours, after which Mef2c expression was examined by qPCR. n = 4 samples. Compared to scrambled control, c indicates p<0.001. (B) UMR106.01 cells were cultured with 50 nM of scrambled or Ptger4 siRNA for 48 hours, after which Mef2d expression was examined by qPCR. n = 4 samples. Compared to scrambled control, c indicates p<0.01. (C) UMR106.01 cells were cultured with 50 nM of scrambled, Mef2c, or Mef2d siRNA for 48 hours, then with 5 µM PGE2 for 3 hours, after which time total RNA was collected and analyzed for Sost and Rpl32. n = 5 samples. Compared to target siRNA control, b indicates p<0.01 and c indicates p<0.001.