Prostaglandin E2 Signals Through PTGER2 to Regulate Sclerostin Expression
Figure 5
Effects of actinomycin D or cycloheximide upon PGE2 suppression of Sclerostin.
(A) UMR106.01 cells were serum-starved for 1 hour, treated with 2.5 µg/mL actinomycin D with or without 5 µM PGE2, and collected 3 hours later. cDNA was prepared for qPCR analysis of Sost and Rpl32. n = 4 samples. (B) UMR 106.01 cells were treated with combinations of 10 µg/mL cycloheximide and 5 µM PGE2 for 3 hours. Samples were analyzed by qPCR for Sost and Rpl32. n = 4 samples. Compared to vehicle control, a indicates p<0.05 and b indicates p<0.001; compared to 5 µM PGE2, c indicates p<0.001.