Prostaglandin E2 Signals Through PTGER2 to Regulate Sclerostin Expression
Figure 4
PGE2 signals through Ptger2 to decrease Sost expression.
(A) Cells were cultured for 3 hours in the presence of PTH (100 nM), PGE2, EP2 agonist butaprost, or EP4 agonist CAY10580 (each 500 nM). Sost expression was analyzed by qPCR and normalized to Rpl32. n = 5 samples. Compared to vehicle control, b indicates p<0.01 and c indicates p<0.001; a indicates p<0.01 versus CAY10580. (B) UMR106.01 cells were cultured with 50 nM of scrambled or Ptger2 siRNA for 48 hours, after which Ptger2 expression was examined by qPCR. n = 4 samples. Compared to vehicle control, a indicates p<0.05. (C) UMR106.01 cells were cultured with 50 nM of scrambled or Ptger4 siRNA for 48 hours, after which Ptger4 expression was examined by qPCR. n = 4 samples. Compared to vehicle control, a indicates p<0.05. (D) UMR106.01 cells were cultured with 50 nM of scrambled, Ptger2, or Ptger4 siRNA for 48 hours, then with 5 µM PGE2 for 3 hours, after which time total RNA was collected and analyzed for Sost and Rpl32. n = 5 samples. Compared to vehicle control, b indicates p<0.01.