Expanding the Versatility of Phage Display II: Improved Affinity Selection of Folded Domains on Protein VII and IX of the Filamentous Phage
Figure 1
Schematic drawing of the filamentous phage structure.
(A) The wt virion is made up of five structural proteins that coat a single stranded DNA genome of about 6.4 kb. (B) In the wt phage there is about 2,700 copies of pVIII and approximately 3–5 copies each of the four proteins pIII, pVI, pVII and pIX, which are found at each tip of the virion [4], [35]. The virion size depends on the genome size at approx. 2.3 nucleotides per pVIII, and hence the length of the particle changes as a function of genome length [36]. The theoretical MW of the mature capsid proteins were calculated from the sequence of VCSM13 (GenBank accession no.: AY598820).