Antigen-Specific Monoclonal Antibodies Isolated from B Cells Expressing Constitutively Active STAT5
Figure 1
STAT5bER immortalized memory B cells down regulate Ig secretion.
A) IgG production in time from STAT5bER transduced memory B cells cultured in the presences of CD40L, IL-2, IL-4 and tamoxifen (4HT) or CD40L, IL-21 and tamoxifen (4HT). Two representative donors out of 10 donors are shown. B) IgM production of peripheral blood B cells transduced with CASTAT5b cultured in the presence of CD40L, IL-2 and IL-4 at day 8 and 65 after transduction. Depicted are data from two donors.