Functional Role of Glutamine 28 and Arginine 39 in Double Stranded RNA Cleavage by Human Pancreatic Ribonuclease
Figure 1
Sequence alignment of HPR with other ribonucleases.
The sequences of ribonucleases were taken from protein data bank their PDB Ids being: HPR, human pancreatic ribonuclease (1DZA); BS-RNase, bovine seminal ribonuclease (1BSR); RNase A: bovine pancreatic ribonuclease (3JW1). The Swiss-Prot ID of DPR, Douc Pancreatic Ribonuclease is Q8SPN4.1. In the parenthesis is given the percent amino acid sequence similarity between different ribonucleases with respect to HPR. The secondary structures are shown at the top of sequences as, α-helices in brown filled box and β-strands in green filled arrow while the loop residues as black line. The identical residues are shown in orange and the conserved cysteine residues are highlighted in light blue. The active site residues, His12 and His119 are shaded in green while the residues under investigation, Gln28, Gly38 and Arg39 in the current study are highlighted in red.