MicroRNA-21 Exhibits Antiangiogenic Function by Targeting RhoB Expression in Endothelial Cells
Figure 3
Inhibition of miR-21 impairs angiogenesis in vitro.
HUVECs were transfected with a LNA-21 or with a LNA control (LNA-Ctrl) and were assessed for migration, tubulogenesis and proliferation after 48 h. A–B. Migration of transfected HUVECs in a scratch-wound assay 16 h after treatment with bFGF (10 ng/ml) and VEGFa (50 ng/ml) (n = 6–12 measurements/condition; n = 3 experiments). C–E. Transfected HUVECs were seeded onto Matrigel in EGM-2 and then allowed to form capillary-like structures for 16 h. Living cells were labeled with calcein-AM. Representative figures are shown in (C). Branching numbers (D) and tube length (E) were quantified with Image J software (n = 5-10 pictures/condition; n = 3 experiments). Data are means with the SD. *p<0.05. F. Proliferation was determined in transfected HUVECs treated with bFGF (10 ng/ml) and VEGFa (50 ng/ml) by measuring BrdU incorporation (n = 3).