Chlamydia Species-Dependent Differences in the Growth Requirement for Lysosomes
Figure 5
Cathepsin proteolytic activity is required for optimal growth of Chlamydia.
(A) LysoTracker™ Red staining of untreated or 75 µM Cathepsin Inhibitor III-treated HEp-2 cells for the indicated times. Bar represents 10 µm. (B) Cathepsin Inhibitor III compromises chlamydial growth. This cathepsin inhibitor was titrated for its effect and was used at 40 µM. For IFU recovery experiments, data were pooled from at least three independent experiments with the average and standard deviation shown in percentage of the untreated control. Two-tailed Student's t tests assuming equal variance were performed for each experiment and p values combined using Fisher's Test for Combining p values. * indicates p<0.0001 for treatments compared to untreated control.