Identification of an Amphipathic Helix Important for the Formation of Ectopic Septin Spirals and Axial Budding in Yeast Axial Landmark Protein Bud3p
Figure 1
Bud3p localizes to ectopic septin spirals and rings upon overexpression.
(A) Bud3p colocalizes with the septin spirals. Cells of strain JGY1783 (a CDC3-mCherry) carrying plasmid pUG36-BUD3 (expresses Bud3p-GFP) were grown on SC-Ura medium and imaged by two-color fluorescence microscopy. (B) Bud3p localizes to the plasma membrane and ectopic rings, short bars, and arcs. Cells of strain YEF3570 (a bud3Δ) carrying plasmid pUG36-BUD3 were imaged. Cells 1 and 2 were focused on the equatorial plane whereas cells 3 and 4 were focused near the top surface. (C) The ectopic Bud3p rings contain the septins. Cells as shown in A were imaged by two-color fluorescence microscopy. Bar, 5 µm.