Complete Killing of Caenorhabditis elegans by Burkholderia pseudomallei Is Dependent on Prolonged Direct Association with the Viable Pathogen
Figure 2
B. pseudomallei diffusible toxins alone are not sufficient to mediate full killing effect.
(A and B) One-day old Glp worms were transferred to individual B. pseudomallei free culture, Human PMC2000 (open diamonds), Human D286 (open triangles), Human R15 (crosses), Human H10 (open circles), Rabbit 2514 (closed squares), Sheep 4523 (closed triangles), Ostrich 9166 (closed circles) and E. coli OP50 (open squares). Graph shows the mean ± SD of three replicates (30 worms/replicate) from a representative of 3 independent experiments. (A) Conditioned NG medium with grown B. pseudomallei on a 0.22 µm pore size sterilized nitrocellulose filter. (B) Filtered culture supernatant of B. pseudomallei supplemented with S-basal medium.