p38 MAPK-Mediated Bmi-1 Down-Regulation and Defective Proliferation in ATM-Deficient Neural Stem Cells Can Be Restored by Akt Activation
Figure 1
Bmi-1 is posttranscriptionally downregulated in Atm-/- NSCs.
(A) Protein samples (30 µg) from Atm+/+ and Atm-/- NSCs were analyzed by Western blotting for phospho-p38, p38, phospho-Akt, Akt, Bmi-1, and p21 expression. (B) Total RNA was purified from Atm+/+ and Atm-/- NSCs and subjected to quantitative RT-PCR analysis for bmi-1, p21, p16, and p19 expression. Gapdh was used as an internal control.