Mucosal Targeting of a BoNT/A Subunit Vaccine Adjuvanted with a Mast Cell Activator Enhances Induction of BoNT/A Neutralizing Antibodies in Rabbits
Figure 5
Ad2 fiber protein enhances the induction of BoNT/A-neutralizing antibodies in nasally immunized rabbits.
Female Dutch Belted rabbits (4 per group) were nasally immunized on days 0, 14, 28 and 91 with the indicated vaccine formulation. A. Serum collected on day 105 was tested for the presence of BoNT/A-neutralizing antibodies using a mouse neutralization assay. a: serum BoNT/A-neutralizing activity significantly greater than the neutralizing activity measured in rabbits nasally immunized with Hcβtre, Hcβtre + CT, Hcβtre + C48/80 or Hcβtre-Ad2F; ANOVA and Tukey's multiple comparison, p<0.05. b: serum BoNT/A-neutralizing activity significantly greater than the neutralizing activity measured in rabbits nasally immunized with Hcβtre, Hcβtre + CT, Hcβtre + C48/80 or Hcβtre-Ad2F; ANOVA and Tukey's multiple comparison, p<0.05. B. Hcβtre-Ad2F is superior to Hcβtre for the induction of BoNT/A neutralizing antibodies when delivered with CT or C48/80 as adjuvants. a: p = 0.0006, Mann-Whitney test.