MCT Expression and Lactate Influx/Efflux in Tanycytes Involved in Glia-Neuron Metabolic Interaction
Figure 4
MCT4 localization in dorsal β1-tanycytes.
A, Low magnification of hypothalamic area using anti-MCT4 antibodies (green), anti-vimentin antibodies (red), and the nuclear stain, TOPRO (blue). B, Low magnification using anti-MCT4 antibodies (green), anti-GFAP antibodies (red), and the TOPRO nuclear stain (blue). C, High magnification showing GFAP-positive processes of β1d-tanycytes in contact with lateral AN neurons. β1d-tanycytes processes were positive for MCT4 and GFAP (D–F). MCT4 was expressed in a number of β1-tanycyte processes, which expressed vimentin (G–I, arrows). AN, arcuate nucleus, III-V: third ventricle, ME: median eminence. Scale bar: A–B, 150 µm; C–I, 50 µM; J–L, 25 µm.