Differential Expression of Rubisco in Sporophytes and Gametophytes of Some Marine Macroalgae
Figure 4
Real-time PCR analysis for the relative expression level (fold change) of rbcL gene in gametophyte and sporophyte of P. yezoensis, P. haitanensis, B. fuscpurpurea and L. japonica.
The cDNA template was amplified and detected by SYBR green using primers of rbcL gene and two reference genes for each alga. A, P. yezoensis. B, P. haitanensis. C, B. fuscopurpurea. D, L. japonica. A-(a), B-(a), C-(a) and D-(a) are the results that use 18S as the reference gene. A-(b) and B-(b) are the results that use GAPDH as the reference gene. C-(b) and D-(b) are the results that use ACTIN as the calibratorreference gene. S, Sporophyte. G, Gametophyte. MG, Male gametophyte. FG, Female gametophyte. Data are the mean value of three independent experiments (±SD).