Molecular Evolutionary Analysis of ABCB5: The Ancestral Gene Is a Full Transporter with Potentially Deleterious Single Nucleotide Polymorphisms
Figure 1
Predicted topological structures of ABCB5.
A. Topological structure of the ABCB5 full transporter. The figure depicts 12 transmembrane alpha helices and 2 nucleotide-binding domains. The 2 ABC_membrane motifs, amino acids 48–339 and 692–968, are highlighted in orange and the 2 ABC_transporter_2 motifs, amino acids 386–622 and 1015–1253, are highlighted in purple. The 2 N-glycosylation sites, amino acids 85–88 and 91–94, which may be important for targeting of the transporter to the membrane, are highlighted in blue. The topological structure was determined using HMMTOP and displayed using the TOPO2 software. Functional motifs were determined using MOTIFSCAN. B. Topology of ABCB5 beta. Topology was determined with HMMTOP and displayed using TOPO2. Diagram depicts 6 transmembrane alpha helices and 2 nucleotide-binding domains. One ABC_membrane domain, amino acids 247–523, is shown in orange and the 2 ABC_transporter_2 domains, amino acids 2–177 and 570–808, are depicted in purple.